GITR / NF-κB-Luciferase Reporter (Luc) - Jurkat Cell Line | 60546

GITR / NF-κB-Luciferase Reporter (Luc) - Jurkat Cell Line | 60546 | Gentaur US, UK & Europe Disrtribition

Category: Immunotherapy/Cell Line

Application: This cell line is suitable for monitoring the activity of NF-kB signaling in response to GITR ligand (GITRL).

Background: Glucocorticoid-induced TNFR-related protein (GITR) is a member of the TNFR superfamily, expressed in a number of cells including T cells, natural killer (NK) cells and antigen-presenting cells (APC). GITRL, GITR’s natural ligand, is expressed mainly by APCs and GITR:GITRL interaction is important for activation of the immune system. Agonistic antibodies targeting GITR are actively being pursued as potential immuno-oncology therapies.

Description: This cell line expresses a surface human GITR (glucocorticoid-induced TNFR family related gene; TNFRSF18; CD357) and an NF-κB luciferase reporter construct that are stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. The cells have been validated using purified human GITRL and anti-GITR neutralizing antibody.

Product Type: Cell Line

Shippement Condition: -80°C