Affinity Media Ligands

Affinity Media Ligands

Affinity media ligands

Schematics of cell imaging methods involving high-and low-affinity ligands for cell surface receptors. a, b Conventional labeling using only a high-affinity ligand (a) or a low-affinity ligand (b). c Labeling using both high-and low-affinity ligands and a bioorthogonal reaction on the cell surface.

 

Affinity media ligands are molecules that are immobilized onto a solid support for use in affinity chromatography. Affinity chromatography is a separation technique used to purify proteins or other biomolecules based on their specific interactions with ligands immobilized on a chromatography matrix.

These ligands have a high affinity for the target molecule, allowing for selective binding and purification. Affinity media ligands can vary widely depending on the target molecule and the desired purification method. Some common types of affinity media ligands include:

Antibodies:

Antibodies are widely used as affinity ligands for the purification of antigens or proteins that specifically bind to the antibody's epitope.

Enzymes:

Enzymes can be used as affinity ligands for the purification of substrates or other proteins that interact with the enzyme.

Receptors:

Cell surface receptors or soluble receptors can be used as affinity ligands for the purification of ligands or other proteins that bind specifically to the receptor.

Metal ions:

Metal ions such as nickel, cobalt, or zinc can be immobilized on a chromatography matrix and used as affinity ligands for the purification of proteins containing specific metal-binding domains, such as polyhistidine tags.

Lectins:

Lectins are proteins that bind specifically to carbohydrate moieties. They can be immobilized on a chromatography matrix and used as affinity ligands for the purification of glycoproteins or other carbohydrate-containing molecules.

Affinity chromatography using media ligands offers high specificity and purity in protein purification processes.

 

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  • Recombinant Protein L, His Tag

    Recombinant Protein L, His Tag | RPL-P3141-1g

    €3,496.00
    Recombinant Protein L, His Tag | RPL-P3141-1g| Gentaur Distribution US, UK & Europe Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig (IgG, IgM, IgA, IgE and IgD) through L chain...
    716-RPL-P3141-1g
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  • Recombinant Protein L, His Tag

    Recombinant Protein L, His Tag | RPL-P3141-2mg

    €244.00
    Recombinant Protein L, His Tag | RPL-P3141-2mg| Gentaur Distribution US, UK & Europe Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig (IgG, IgM, IgA, IgE and IgD) through L chain...
    716-RPL-P3141-2mg
    €244.00
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  • Recombinant Protein L, His Tag

    Recombinant Protein L, His Tag | RPL-P3141-10mg

    €460.00
    Recombinant Protein L, His Tag | RPL-P3141-10mg| Gentaur Distribution US, UK & Europe Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig (IgG, IgM, IgA, IgE and IgD) through L chain...
    716-RPL-P3141-10mg
    €460.00
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  • Recombinant Protein G, His Tag

    Recombinant Protein G, His Tag | RPG-S3140-1g

    €3,136.00
    Recombinant Protein G, His Tag | RPG-S3140-1g| Gentaur Distribution US, UK & Europe Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa...
    716-RPG-S3140-1g
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  • Recombinant Protein G, His Tag

    Recombinant Protein G, His Tag | RPG-S3140-2mg

    €208.00
    Recombinant Protein G, His Tag | RPG-S3140-2mg| Gentaur Distribution US, UK & Europe Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa...
    716-RPG-S3140-2mg
    €208.00
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  • Recombinant Protein G, His Tag

    Recombinant Protein G, His Tag | RPG-S3140-10mg

    €304.00
    Recombinant Protein G, His Tag | RPG-S3140-10mg| Gentaur Distribution US, UK & Europe Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a...
    716-RPG-S3140-10mg
    €304.00
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