NF-κB Reporter (Luc) - HCT116 Cell Line | 60623

NF-κB Reporter (Luc) - HCT116 Cell Line | 60623 | Gentaur US, UK & Europe Disrtribition

Category: Cell Signaling/Cell Line

Application: N/A

Background: N/A

Description: NF-
B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The
firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of
the TATA promoter. Following activation by stimulants, endogenous NF-κ
B transcription factors
bind to the DNA response elements to induce transcription of the luciferase gene. The NF-
κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-κB signaling
in response to stimulants such as the cytokines TNF and IL-1β, pathogen-associated
molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern
(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for
establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules.
This cell line can be further modified to allow investigation of downstream NF-κB activities as a
result of targeted genetic mutation(s).

Product Type: Cell Line

Shippement Condition: dry ice