NCI-H358 Cell Line | CL-0400

NCI-H358 Cell Line | CL-0400 | Gentaur US, UK & Europe Disrtribition

Media: CM-0400

Organism: Homo sapiens, Human

Application: This line was derived by A.F.Gazdar, H.K.Oie, J.D.Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Cla

Background: N/A

Age: N/A

Tissue: Lung/bronchiole; Derived from metastatic site: alveolus

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: ~38-60 hours

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:4

Renewal: every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+32% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

Effects: Yes, the cells produce tumors in athymic nude mice.

Duration: N/A

Lead Time: 3-5 days