NCI-H69 Cell Line | CL-0677

NCI-H69 Cell Line | CL-0677 | Gentaur US, UK & Europe Disrtribition

Media: CM-0677

Organism: Homo sapiens, Human

Application: N/A

Background: This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired.

Age: 55 years

Tissue: Pleural effusion

Morphology: Floating aggregates

Growth Properties: Suspension, multicellular aggregates

Doubling Time: 56.0 hours

Biosafty: 1

Medium: RPMI-1640(PM150110)+10%FBS+1%P/S(PB180120)

Subculturing: Attached cells can be detached by shaking flask Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Do not break down aggregates. Subculture every 6 to 8 days.

Ratio: 1:2 to 1:4

Renewal: 2 times per week

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days