MBP Antibody / Myelin Basic Protein | V8437-100UG

MBP Antibody / Myelin Basic Protein | V8437-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Format: Purified

Clone: rMBP/4288

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG2a, kappa

Species Reactivity: Human

Application: WB, IHC-P

Application Details: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT

Application Note: Optimal dilution of the recombinant MBP antibody should be determined by the researcher.

Purity: Protein G affinity chromatography

Description: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas.

Immunogen: A portion of amino acids 150-250 from the human protein was used as the immunogen for the recombinant MBP antibody.

Storage: Store the recombinant MBP antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cell surface, cytoplasm