GIPR/CRE Luciferase Reporter HEK293 Cell Line | 78589

GIPR/CRE Luciferase Reporter HEK293 Cell Line | 78589 | Gentaur US, UK & Europe Disrtribition

Category: Cell Signaling/Cell Line

Application: Screen and characterize agonists of human GIPR in a cellular context

Background: The gastric inhibitory polypeptide receptor (GIPR), also known as the glucose-dependent insulinotropic polypeptide receptor belongs to the Class B1 G protein-coupled receptor (GPCR) family. GIPR is primarily found in the β-cells of the pancreas and serves as a receptor for the gastric inhibitory polypeptide (GIP) hormone. As one of the incretin hormones, GIP modulates glucose metabolism by stimulating the pancreatic β-cells to release insulin. Since GIPR/GLP-1R heterodimerization has been shown to regulate GLP-1R signaling, dual agonists that bind both GIPR and GLP-1R have shown promising clinical efficacy for treating type II diabetes mellitus (T2DM) and obesity.  

Description: Recombinant HEK293 cells expressing the firefly luciferase gene under the control of cAMP response element (CRE), and with forced expression of human GIPR (Gastric Inhibitory Polypeptide receptor; NM_000164.4). Activation of GIPR in these cells can be monitored by measuring luciferase activity. The functionality of the GIPR/CRE Luciferase Reporter HEK293 Cell Line was validated in a dose-response assay using agonists gastric inhibitory peptide (GIP) and tirzepatide hydrochloride. These agonists induce luciferase activity in a dose-dependent manner as depicted in Figure 1.

Figure 1. Illustration of the GIPR/CRE Luciferase Reporter HEK293 Cell line.

Product Type: Cell Line

Shippement Condition: -80°C