AAV5 Luciferase-mCherry | 78474

AAV5 Luciferase-mCherry | 78474 | Gentaur US, UK & Europe Disrtribition

Category: Viral Tools/AAV

Application: Use as a positive control for transduction Optimize transduction assays and track protein expression over time

Background: N/A

Description: Adeno-Associated Virus Serotype 5 (AAV5) differs from other parvovirus serotypes according to serological and DNA hybridization data, and AAV5 also uses different inverted terminal repeats (ITRs) compared to other AAV serotypes. AAV5 is the most efficient vector for transducing sensory neurons and is good at mediating gene transfer into human and murine airway epithelia. In addition, AAV5 vectors show a higher tropism for both mouse and human dendritic cells than AAV1, AAV2, AAV7, or AAV8. These AAV particles constitutively express the firefly (Photinus pyralis) luciferase and mCherry genes connected via a T2A linker, under the control of a CMV promoter. The T2A self-cleaving peptide (derived from Thosea asigna virus 2A) leads to the efficient cleavage of the transcript, and expression of luciferase and mCherry as two separate proteins.

Product Type: Viral Tools

Shippement Condition: -80°C