VA(Vitamin A) ELISA Kit | E-EL-0135

VA(Vitamin A) ELISA Kit | E-EL-0135 | Gentaur US, UK & Europe Disrtribition

Target Species: Universal

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivitys: 9.38ng/mL

Detection Range: 15.63~1000ng/mL

Target Name: VA

Target Synonyms: VA

Tested Sample: Serum, plasma and other biological fluids

Test Principale: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VA. During the reaction, VA in the sample or standard competes with a fixed amount of VA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes VA in samples. No significant cross-reactivity or interference between VA and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of VA concentrations in serum, plasma and other biological fluids.

Research Area: N/A

Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.