TBP Polyclonal Antibody | E-AB-18293

TBP Polyclonal Antibody | E-AB-18293 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human

Host: Rabbit

Isotype: IgG

Application: WB, ELISA

Research Area: Cancer, Epigenetics and Nuclear Signaling

Background: The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).

Lead Time: 7~10 days

Concentration: 0.8 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Full length fusion protein

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Purification: Antigen affinity purification

Dilution: WB 1:500-1:2000, ELISA 1:5000-1:10000

Conjugation: Unconjugated

Calculated Molecular Weight: 38 kDa

Observed Molecular Weight: Refer to figures

Clonality: Polyclonal