Description
SV-HUC-1 Cell Line | CL-0222 | Gentaur US, UK & Europe Disrtribition
Media: CM-0222
Organism: Homo sapiens, Human
Application: N/A
Background: The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative. Stress(such as chemical exposure) could possibly activate the virus.
Age: 11 years
Tissue: Ureter, uroepithelium
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 2 [Cells contain Adenovirus]
Medium: Ham's F-12K(PM150910)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: No, in nude mice.
Duration: N/A
Lead Time: 3-5 days
