SJSA-1 Cell Line | CL-0703

SJSA-1 Cell Line | CL-0703 | Gentaur US, UK & Europe Disrtribition

Media: CM-0703

Organism: Homo sapiens, Human

Application: N/A

Background: The SJSA-1 cell line (formerly OsA-CL) was established in 1982 from the primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur.

Age: 20 years

Tissue: Bone

Morphology: fibroblast

Growth Properties: Adherent

Doubling Time: 31 hours

Biosafty: 1

Medium: RPMI-1640(PM150110)+10%FBS+1%P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:5 to 1:10

Renewal: 2 to 3 times per week

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days