Description
SJSA-1 Cell Line | CL-0703 | Gentaur US, UK & Europe Disrtribition
Media: CM-0703
Organism: Homo sapiens, Human
Application: N/A
Background: The SJSA-1 cell line (formerly OsA-CL) was established in 1982 from the primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur.
Age: 20 years
Tissue: Bone
Morphology: fibroblast
Growth Properties: Adherent
Doubling Time: 31 hours
Biosafty: 1
Medium: RPMI-1640(PM150110)+10%FBS+1%P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:5 to 1:10
Renewal: 2 to 3 times per week
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
