SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.86/Denmark/EPI_ISL_18114953+H69V70) (MALS verified) | SPN-C5220-50ug

SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.86/Denmark/EPI_ISL_18114953+H69V70) (MALS verified) | SPN-C5220-50ug| Gentaur Distribution US, UK & Europe

It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source: SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.86/Denmark/EPI_ISL_18114953+H69V70) (SPN-C5220) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (ins16MPLF, T19I, R21T, LPP24-26Del, A27S, S50L, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, N460K, S477N, T478K, N481K, V483del, E484). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2.86/Denmark/EPI_ISL_18114953+H69V70). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Format: Powder.

Tag: C-10×His.

Expression System: HEK293.

Expression Region: Val 16 - Pro 1213.

Conjugate: Unconjugated.

Molecular Weight: 138.3 kDa.

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.3 kDa. The protein migrates as 180.0 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Purity: 90%.

Buffer: PBS.

Storage Conditions: -20℃.

Shipping Conditions: RT