Description
RG2 [D74] cell line | CL-0641 | Gentaur US, UK & Europe Disrtribition
Media: CM-0641
Organism: Rattus norvegicus, rat
Application: N/A
Background: RG2 was submitted to the American Type Culture Collection in March, 1999 at passage 3 from a culture established from a tumor. This tumor was produced by Drs. A. Koestner and W. Wechsler at The Ohio State University in 1971.
Age: N/A
Tissue: Brain
Morphology: Glial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 1
Medium: DMEM(PM150210)+10%FBS+1%P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:5
Renewal: Every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days