Recombinant SARS-CoV Nucleoprotein / NP Protein (His Tag) | PKSV030248

Recombinant SARS-CoV Nucleoprotein / NP Protein (His Tag) | PKSV030248 | Gentaur US, UK & Europe Disrtribition

Synonyms: coronavirus NP Protein, SARS; coronavirus Nucleocapsid Protein, SARS; coronavirus Nucleoprotein Protein, SARS; cov np Protein, SARS; ncov NP Protein, SARS; novel coronavirus NP Protein, SARS; novel coronavirus Nucleocapsid Protein, SARS; novel coronavirus Nucleoprotein Protein, SARS; NP Protein, SARS; Nucleocapsid Protein, SARS; Nucleoprotein Protein, SARS

Active Protein: N/A

Activity: A DNA sequence encoding the SARS-CoV (isolate:Tor2) nucleoprotein (NP_828858.1) (Met1-Ala422) was expressed with a C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the SARS-CoV (isolate:Tor2) nucleoprotein (NP_828858.1) (Met1-Ala422) was expressed with a C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: SARS

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 80 % as determined by reducing SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 47.5kDa

Formulation: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Research Area: Virus related Protein