Recombinant Mouse SMPD1/ASM Protein (His Tag)(Active) | PKSM040504

Recombinant Mouse SMPD1/ASM Protein (His Tag)(Active) | PKSM040504 | Gentaur US, UK & Europe Disrtribition

Synonyms: A-SMase;ASM;aSMase;Zn-SMase

Active Protein: Active protein

Activity: A DNA sequence encoding the mouse SMPD1 (Q04519) (Met 1-Leu 626) was expressed，with a C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the mouse SMPD1 (Q04519) (Met 1-Leu 626) was expressed，with a C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: Mouse

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 66.3 kDa

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 0.1% Tween20

Reconstitution: Please refer to the printed manual for detailed information.

Background: Sphingomyelin phosphodiesterase 1 (SMPD1), also known as ASM ( acid sphingomyelinase ), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86% aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid ) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.

Research Area: N/A