Recombinant Mouse Siglec-F (C-6His) | PKSM041438

Recombinant Mouse Siglec-F (C-6His) | PKSM041438 | Gentaur US, UK & Europe Disrtribition

Synonyms: SAF2; sialic acid binding Ig like lectin 8; SIGLEC-8; SIGLEC8L; SiglecF; Siglec-F

Active Protein: N/A

Activity: Recombinant Mouse Sialic Acid-binding Ig-like Lectin 5 is produced by our Mammalian expression system and the target gene encoding Asp18-Thr437 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Mouse Sialic Acid-binding Ig-like Lectin 5 is produced by our Mammalian expression system and the target gene encoding Asp18-Thr437 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Mouse

Expressed Host: Human Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 46.2 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Siglec 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system. This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Mouse Siglec-F cDNA encodes a 569 amino acid polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. The expression of Siglec-F is restricted to the cells of myelomonocytic lineage. Mouse Siglec-F is likely an ortholog of human Siglec-5. Unlike many human CD33-related Siglecs, which show similar binding to both alpha 2, 3- and alpha 2, 6-linked sialic acids, mouse Siglec-F preferentially recognize alpha 2, 3-linked sialic acid.

Research Area: N/A