Description
Recombinant Mouse Carboxypeptidase B1/CPB1 Protein (His Tag)(Active) | PKSM040708 | Gentaur US, UK & Europe Disrtribition
Synonyms: 0910001A18Rik;1810063F02Rik;2210008M23Rik;AI504870
Active Protein: Active protein
Activity: A DNA sequence encoding the mouse CPB1 (NP_083982.1) precursor (Met 1-Tyr 415) was expressed, with a C-terminal polyhistidine tag.
Protein Construction: A DNA sequence encoding the mouse CPB1 (NP_083982.1) precursor (Met 1-Tyr 415) was expressed, with a C-terminal polyhistidine tag.
Fusion Tag: C-His
Species: Mouse
Expressed Host: HEK293 Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 94 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 47.5 kDa
Formulation: Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.5
Reconstitution: Please refer to the printed manual for detailed information.
Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Research Area: N/A
