Recombinant Human Carboxypeptidase B1/CPB1 Protein (His Tag)(Active) | PKSH031567

Recombinant Human Carboxypeptidase B1/CPB1 Protein (His Tag)(Active) | PKSH031567 | Gentaur US, UK & Europe Disrtribition

Synonyms: Carboxypeptidase B; Pancreas-Specific Protein; PASP; CPB1; CPB; PCPB

Active Protein: Active protein

Activity: A DNA sequence encoding the human CPB1 (NP_001862.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the human CPB1 (NP_001862.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 98 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 47 kDa

Formulation: Lyophilized from sterile 25mM MES, 0.1 M NaCl, pH 6.5

Reconstitution: Please refer to the printed manual for detailed information.

Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Research Area: Signal Transduction, metabolism,