Recombinant Human PBEF/NAMPT Protein (His & GST Tag) | PKSH031981

Recombinant Human PBEF/NAMPT Protein (His & GST Tag) | PKSH031981 | Gentaur US, UK & Europe Disrtribition

Synonyms: Pre-B cell-enhancing factor; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Visfatin; NAMPT; PBEF; PBEF1

Active Protein: N/A

Activity: A DNA sequence encoding the human NAMPT (P43490) (Met 1-His 491 was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human NAMPT (P43490) (Met 1-His 491 was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Fusion Tag: N-His & GST

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 83.3 kDa

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 20% gly, 0.3mM DTT 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please c

Reconstitution: Please refer to the printed manual for detailed information.

Background: Nicotinamide phosphoribosyltransferase (NAMPT); also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin; is an enzyme belonging to the family of glycosyltransferases; to be specific; the pentosyltransferases. This enzyme participates in nicotinate and nicotinamide metabolism. This enzyme catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1- pyrophosphate to yield nicotinamide mononucleotide; one step in the biosynthesis of nicotinamide adenine dinucleotide. NAMPT is also considered as an essential enzyme mediating granulocyte colony-stimulating factor (G-CSF)-triggered granulopoiesis in healthy individuals and in individuals with severe congenital neutropenia. Intracellular NAMPT and NAD+ amounts in myeloid cells; as well as plasma NAMPT and NAD+ levels; were increased by G-CSF treatment of both healthy volunteers and individuals with congenital neutropenia.

Research Area: Signal Transduction, Cardiovascular, Neuroscience, Cancer, immunology, metabolism,