Recombinant Human KEAP1/INRF2 Protein (His & GST & AVI Tag) | PKSH030944

Recombinant Human KEAP1/INRF2 Protein (His & GST & AVI Tag) | PKSH030944 | Gentaur US, UK & Europe Disrtribition

Synonyms: INRF2;KEAP-1;KLHL19

Active Protein: N/A

Activity: A DNA sequence encoding the human KEAP1 (Q14145) (Gln 2-Cys 624) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus linked by the AVI tag.

Protein Construction: A DNA sequence encoding the human KEAP1 (Q14145) (Gln 2-Cys 624) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus linked by the AVI tag.

Fusion Tag: N-His & GST & AVI

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 99.2 kDa

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: Kelch-like ECH-associated protein 1; also known as cytosolic inhibitor of Nrf2; Kelch-like protein 19; KEAP1 and INRF2; is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain; one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed; with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor; which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions; KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome; thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.

Research Area: epigenetics and nuclear signal