Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155)(Q65I, C96S, N111G) | PKSH033502

Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155)(Q65I, C96S, N111G) | PKSH033502 | Gentaur US, UK & Europe Disrtribition

Synonyms: Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB

Active Protein: N/A

Activity: Recombinant Human FGF-2(Q65I;C96S;N111G) is produced by our E.coli expression system and the target gene encoding Met1-Ser155 is expressed.

Protein Construction: Recombinant Human FGF-2(Q65I;C96S;N111G) is produced by our E.coli expression system and the target gene encoding Met1-Ser155 is expressed.

Fusion Tag: N/A

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 17.2 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH7.5.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro; including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable; daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule; so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies; and maintained cell growth; pluripotency and differentiation potential with a 2-day feeding schedule.

Research Area: N/A