Recombinant E.coli Lactose Operon Repressor/LacI Protein (His Tag) | PKSQ050058

Recombinant E.coli Lactose Operon Repressor/LacI Protein (His Tag) | PKSQ050058 | Gentaur US, UK & Europe Disrtribition

Synonyms: Lactose operon repressor; LacI

Active Protein: N/A

Activity: Recombinant E.coli Lactose operon repressor is produced by our E.coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant E.coli Lactose operon repressor is produced by our E.coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-His

Species: E.coli

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 39.4 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0.

Reconstitution: Not Applicable

Background: Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.

Research Area: N/A