Recombinant Cynomolgus TIGIT/VSIG9/VSTM3 Protein (Fc Tag) | PKSQ050021

Recombinant Cynomolgus TIGIT/VSIG9/VSTM3 Protein (Fc Tag) | PKSQ050021 | Gentaur US, UK & Europe Disrtribition

Synonyms: T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; TIGIT; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9

Active Protein: N/A

Activity: Recombinant Cynomolgus TIGIT is produced by our Mammalian expression system and the target gene encoding Met89-Pro209 is expressed with a Fc tag at the C-terminus.

Protein Construction: Recombinant Cynomolgus TIGIT is produced by our Mammalian expression system and the target gene encoding Met89-Pro209 is expressed with a Fc tag at the C-terminus.

Fusion Tag: C-Fc

Species: Cynomolgus

Expressed Host: Human Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 40.6 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Reconstitution: Please refer to the printed manual for detailed information.

Background: T cell immunoreceptor with Ig and ITIM domains (TIGIT), also called VSIG9 and Vstm3, is a member of the CD28 family within the Ig superfamily of proteins. TIGIT contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM), and is expressed on regulatory, memory, activated T cells and NK cells. TIGIT binds to CD155(PVR) that appear on dendritic cells (DC), macrophages and endothelium with high affinity, and CD112(PVRL2) with lower affinity, but not CD113 (PVRL3). TIGIT-Fc fusion protein could interact with PVR on DC and enhance the secretion of IL-10, but inhibit the macrophage activation. Mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges, while knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses.

Research Area: N/A