QuicKey Human VEGF-A(Vascular Endothelial Growth Factor A) ELISA Kit | E-TSEL-H0026

QuicKey Human VEGF-A(Vascular Endothelial Growth Factor A) ELISA Kit | E-TSEL-H0026 | Gentaur US, UK & Europe Disrtribition

Target Species: Human

Type: Sandwich

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivitys: 0.03ng/mL

Detection Range: 0.04~10ng/mL

Target Name: VEGF-A

Target Synonyms: VEGF-A, VEGFA, MVCD1, VPF

Tested Sample: Serum and plasma

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-A. Samples (or Standards) and biotinylated detection antibody specific for Human VEGF-A are added to the micro ELISA plate wells. Human VEGF-A would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human VEGF-A. You can calculate the concentration of Human VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Human VEGF-A in samples. No significant cross-reactivity or interference between Human VEGF-A and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Human VEGF-A concentrations in Serum and plasma.

Research Area: Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction

Storage : An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.