QuicKey Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit | E-TSEL-H0003

QuicKey Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit | E-TSEL-H0003 | Gentaur US, UK & Europe Disrtribition

Target Species: Human

Type: Sandwich

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivitys: 0.06ng/mL

Detection Range: 0.13~8ng/mL

Target Name: NGAL

Target Synonyms: N/A

Tested Sample: Serum, Plasma, Urine

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated withan antibody specific to Human NGAL. Samples (or Standards) and biotinylateddetection antibody specific for Human NGAL are added to the micro ELISA platewells. Human NGAL would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to eachmicro plate well and incubated. Free components are washed away. The substratesolution is added to each well. Only those wells that contain Human NGAL, biotinylateddetection antibody and Avidin-HRP conjugate will appear blue in color. Theenzyme-substrate reaction is terminated by the addition of stop solution andthe color turns yellow. The optical density (OD) is measuredspectrophotometrically at a wavelength of 450 ± 2 nm. The OD value isproportional to the concentration of Human NGAL. You can calculate theconcentration of Human NGAL in the samples by comparing the OD of the samplesto the standard curve.

Specificity: This kit recognizes Human NGAL in samples. No significant cross-reactivity or interference between Human NGAL and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Human NGAL concentrations in Serum, Plasma, Urine .

Research Area: Cancer, Cardiovascular, Metabolism, Immunology, Signal transduction

Storage : An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.