Description
QuicKey Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit | E-TSEL-H0003 | Gentaur US, UK & Europe Disrtribition
Target Species: Human
Type: Sandwich
Assay Time: 2.5h
Detection Type: Colormetric
Sensitivitys: 0.06ng/mL
Detection Range: 0.13~8ng/mL
Target Name: NGAL
Target Synonyms: N/A
Tested Sample: Serum, Plasma, Urine
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated withan antibody specific to Human NGAL. Samples (or Standards) and biotinylateddetection antibody specific for Human NGAL are added to the micro ELISA platewells. Human NGAL would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to eachmicro plate well and incubated. Free components are washed away. The substratesolution is added to each well. Only those wells that contain Human NGAL, biotinylateddetection antibody and Avidin-HRP conjugate will appear blue in color. Theenzyme-substrate reaction is terminated by the addition of stop solution andthe color turns yellow. The optical density (OD) is measuredspectrophotometrically at a wavelength of 450 ± 2 nm. The OD value isproportional to the concentration of Human NGAL. You can calculate theconcentration of Human NGAL in the samples by comparing the OD of the samplesto the standard curve.
Specificity: This kit recognizes Human NGAL in samples. No significant cross-reactivity or interference between Human NGAL and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human NGAL concentrations in Serum, Plasma, Urine .
Research Area: Cancer, Cardiovascular, Metabolism, Immunology, Signal transduction
Storage : An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
