P815 Cell Line | CL-0182

P815 Cell Line | CL-0182 | Gentaur US, UK & Europe Disrtribition

Media: CM-0182

Organism: Mus musculus, Mouse

Application: Transfection host.

Background: P815 cells phagocytose latex beads but not zymosan or BCG.They do not function in antibody dependent cell mediated cytotoxicity.Growth of the cells is not inhibited by dextran sulfate, LPS or PPD.Tested and found negative for ectromelia virus(mousepox).

Age: N/A

Tissue: Mastocytoma

Morphology: Rounded

Growth Properties: Mixed, Adherent And Suspension

Doubling Time: ~18-22 hours

Biosafty: 1

Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days