Description
NCI-H69 Cell Line | CL-0677 | Gentaur US, UK & Europe Disrtribition
Media: CM-0677
Organism: Homo sapiens, Human
Application: N/A
Background: This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired.
Age: 55 years
Tissue: Pleural effusion
Morphology: Floating aggregates
Growth Properties: Suspension, multicellular aggregates
Doubling Time: 56.0 hours
Biosafty: 1
Medium: RPMI-1640(PM150110)+10%FBS+1%P/S(PB180120)
Subculturing: Attached cells can be detached by shaking flask Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Do not break down aggregates. Subculture every 6 to 8 days.
Ratio: 1:2 to 1:4
Renewal: 2 times per week
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
