Description
NCI-H524 cell line | CL-0403 | Gentaur US, UK & Europe Disrtribition
Media: CM-0403
Organism: Homo sapiens, Human
Application: N/A
Background: The line was established in 1982 from metastatic lymph nodes of a 63-year-old male Caucasian smoker with non-small cell lung carcinoma. The patient received prior chemotherapy and radiation therapy.
Age: male, 63 years
Tissue: Lung; Originating From Metastatic Sites: Lymph Nodes
Morphology: Rounded
Growth Properties: Suspension
Doubling Time: ~100 hours
Biosafty: 1
Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
Ratio: 1×10^5 to 1×10^6 cells/mL
Renewal: 2 to 3 times per week
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
