NCI-H209 [H209] cell line | CL-0393

NCI-H209 [H209] cell line | CL-0393 | Gentaur US, UK & Europe Disrtribition

Media: CM-0393

Organism: Homo sapiens, Human

Application: N/A

Background: This cell line was established in 1979 from the bone marrow aspirated of a 55-year-old male Caucasian with small cell lung carcinoma prior to treatment. The line is a classic small cell lung carcinoma cell line that expresses four biochemical markers, i.e., neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.

Age: 55 years

Tissue: Lung; Originating From Metastatic Site: Bone Marrow

Morphology: Round Cell Clusters

Growth Properties: Suspension

Doubling Time: ~50-70 hours

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:3.

Ratio: 1:2 to 1:3

Renewal: 2 to 3 times per week

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days