NCI-H1568 [H1568] Cell Line | CL-0690

NCI-H1568 [H1568] Cell Line | CL-0690 | Gentaur US, UK & Europe Disrtribition

Media: CM-0690

Organism: Homo sapiens, Human

Application: N/A

Background: The line was established in December 1986 from lymph node metastasis of lung.

Age: 48 years

Tissue: Lymph node

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: 42.5 hours

Biosafty: 1

Medium: RPMI-1640(PM150110)+10%FBS+1%P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:6

Renewal: every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days