NB4 Cell Line | CL-0676

NB4 Cell Line | CL-0676 | Gentaur US, UK & Europe Disrtribition

Media: CM-0676

Organism: Homo sapiens, Human

Application: N/A

Background: established from the bone marrow of a 23-year-old woman with acute promyelocytic leukemia (APL = AML FAB M3) in second relapse in 1989; a method of NB-4 transfection was patented in 2016; cells carry the t(15;17) PML-RARA fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

Age: Female, 23 years

Tissue: Bone

Morphology: lymphoblast-like

Growth Properties: Suspension

Doubling Time: 36-40 hours (PubMed=1995093); 30 hours (PubMed=25984343); ~35-45 hours (DSMZ).

Biosafty: 1

Medium: RPMI-1640 (PM150110)+10% FBS+1% P/S (PB180120)

Subculturing: N/A

Ratio: N/A

Renewal: every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+68% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days