Description
NB4 Cell Line | CL-0676 | Gentaur US, UK & Europe Disrtribition
Media: CM-0676
Organism: Homo sapiens, Human
Application: N/A
Background: established from the bone marrow of a 23-year-old woman with acute promyelocytic leukemia (APL = AML FAB M3) in second relapse in 1989; a method of NB-4 transfection was patented in 2016; cells carry the t(15;17) PML-RARA fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
Age: Female, 23 years
Tissue: Bone
Morphology: lymphoblast-like
Growth Properties: Suspension
Doubling Time: 36-40 hours (PubMed=1995093); 30 hours (PubMed=25984343); ~35-45 hours (DSMZ).
Biosafty: 1
Medium: RPMI-1640 (PM150110)+10% FBS+1% P/S (PB180120)
Subculturing: N/A
Ratio: N/A
Renewal: every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+68% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
