Mouse INS(Insulin) ELISA Kit | E-EL-M2614

Mouse INS(Insulin) ELISA Kit | E-EL-M2614 | Gentaur US, UK & Europe Disrtribition

Target Species: Mouse

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivitys: 0.19ng/mL

Detection Range: 0.31~20ng/mL

Target Name: INS

Target Synonyms: IDDM2, ILPR, IRDN, MODY10

Tested Sample: Cell culture supernatant and tissue homogenate

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in Cell culture supernatant and tissue homogenate.

Research Area: Cancer

Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.