MC3T3-E1 Subclone 14 Cell Line | CL-0378

MC3T3-E1 Subclone 14 Cell Line | CL-0378 | Gentaur US, UK & Europe Disrtribition

Media: CM-0378

Organism: Mus musculus, Mouse

Application: These cell lines are good models for studying in vitro osteoblast differentiation, particularly ECM signaling. They have behavior similar to primary calvarial osteoblasts.

Background: The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. The MC3T3-E1 Subclone 4 and the MC3T3 Subclone 14 lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4mM inorganic phosphate.

Age: Newborn

Tissue: Bone/calvaria

Morphology: Fibroblast

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: MEMα(PM150421)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in immunodeficient mice.

Duration: N/A

Lead Time: 3-5 days