M-07e Cell Line | CL-0686

M-07e Cell Line | CL-0686 | Gentaur US, UK & Europe Disrtribition

Media: CM-0686

Organism: Homo sapiens, Human

Application: N/A

Background: Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

Age: 6 months

Tissue: Peripheral blood

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: 46 +- 12 hours (PubMed=9096695); ~40 hours (DSMZ).

Biosafty: 1

Medium: RPMI-1640(PM150110)+10%FBS+8ng/mlGM-CSF+1%P/S(PB180120)

Subculturing: N/A

Ratio: 1:2 to 1:3

Renewal: Every 2 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days