L-WRN Cell Line | CL-0658

L-WRN Cell Line | CL-0658 | Gentaur US, UK & Europe Disrtribition

Media: CM-0658

Organism: Mus Musculus, Mouse

Application: N/A

Background: The L-WRN cells were derived by transfecting L-Wnt3A with an R-spondin 3 and noggin co-expressing vector and stable clones were selected in medium containing G418 and Hygromycin B.

Age: 100 days

Tissue: Subcutaneous connective tissue; areolar and adipose

Morphology: Fibroblast

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 2

Medium: DMEM (PM150210)+0.4mg/mL G418 (PB180125)+10% FBS+1% P/S (PB180120)

Subculturing: N/A

Ratio: 1:3 to 1:4

Renewal: every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+66% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days