L Wnt-3A Cell Line | CL-0375

L Wnt-3A Cell Line | CL-0375 | Gentaur US, UK & Europe Disrtribition

Media: CM-0375

Organism: Mus musculus, Mouse

Application: This cell line is presently the best source for production of Wnt-3A conditioned medium.

Background: The Wnt-3A gene encodes a secreted glyoprotein with a variety of signaling effects. Wnt genes control many of the patterning and growth events during embryonic development. The cells secrete biologically active Wnt-3A protein. They are presently the best source for production of Wnt-3A conditioned medium. Since the conditioned medium contains other factors besides the Wnt-3A protein, it is necessary to control any experiments involving the Wnt-3A conditioned medium with control conditioned medium from the parental cell line.

Age: 100 days

Tissue: Subcutaneous connective tissue, areolar and adipose

Morphology: Fibroblast

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: DMEM(PM150210)+0.4mg/ml G418(PB180125)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days