KYSE-30 cell line | CL-0577

KYSE-30 cell line | CL-0577 | Gentaur US, UK & Europe Disrtribition

Media: CM-0577

Organism: Homo sapiens, Human

Application: N/A

Background: This cell line was established from the mucosal surface of a well differentiated invasive esophageal squamous cell carcinoma from a 64-year-old Japanese male prior to treatment. The cell line was used for heterotransplanting tumours to athymic mice. The cells have a p53 mutation at the splice acceptor site and amplification of cERB B, MYC and CYCLIN D1.

Age: male, 64 years

Tissue: Esophageal Squamous Epithelium

Morphology: Epithelial-Like, With Long Pseudopod

Growth Properties: Adherent

Doubling Time: ~20-30 hours

Biosafty: 1

Medium: RPMI-1640(PM150110)+Ham's F-12(PM150810)+2mM L-Glutamine(PB180420)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:5

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days