Description
KYSE-150 cell line | CL-0638 | Gentaur US, UK & Europe Disrtribition
Media: CM-0638
Organism: Homo sapiens, human
Application: N/A
Background: This cell line was established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures).
Age: Female,49 years
Tissue: Esophageal Squamous Cell Carcinoma
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: ~25 hours
Biosafty: 1
Medium: RPMI-1640(PM150110)+Ham's F-12(PM150810)+10% FBS+1%P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:5
Renewal: Every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
