KI67 Monoclonal Antibody | E-AB-22027

KI67 Monoclonal Antibody | E-AB-22027 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human, Mouse, Rat

Host: Mouse

Isotype: IgG

Application: IHC-p, IF

Research Area: Cancer, Cell Biology, Neuroscience, Tags and Cell Markers

Background: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Lead Time: 7~10 days

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthetic Peptide

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification: Protein A purification

Dilution: IHC 1:100-1:300, IF 1:50-200

Conjugation: Unconjugated

Calculated Molecular Weight: N/A

Observed Molecular Weight: N/A

Clonality: Monoclonal