Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag | IL2-H4210-1mg

Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag | IL2-H4210-1mg| Gentaur Distribution US, UK & Europe

IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer  (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma  inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma  gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer  (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL‑12, produced by macrophages in response to infectious agents, is a central mediator of the cell‑mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

Source: Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (IL2-H4210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A) (Accession # P29460-1 (IL12B) & P29459-1 (IL12A) ).

Format: Powder.

Tag: C-10×His | C-Flag.

Expression System: HEK293.

Expression Region: Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A).

Conjugate: Unconjugated.

Molecular Weight: 36.6 kDa (IL12B) and 23.8 kDa (IL12A).

Characteristics: Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 36.6 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with polyhistidine tag at the C-terminus and subunit IL-12A is fused with flag tag at the C-terminus. The reducing (R) heterodimer protein migrates as 45 kDa (IL12B) and 38-43 kDa (IL12A) respectively due to glyosylation.

Purity: 90%.

Buffer: PBS, pH7.4.

Storage Conditions: -20℃.

Shipping Conditions: RT