Description
Human HMGB-1(High Mobility Group Protein B1) ELISA Kit | E-EL-H1554 | Gentaur US, UK & Europe Disrtribition
Target Species: Human
Type: Sandwich
Assay Time: 3.5h
Detection Type: Colormetric
Sensitivitys: 18.75pg/mL
Detection Range: 31.25~2000pg/mL
Target Name: HMGB-1
Target Synonyms: HMGB1, HMG1, HMG3, SBP-1, Amphoterin
Tested Sample: Serum, plasma and other biological fluids
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in Serum, plasma and other biological fluids.
Research Area: Cancer, Epigenetics and Nuclear Signaling
Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
