Human Glucosylceramidase Protein, His Tag | GLE-H52H3-1mg

Human Glucosylceramidase Protein, His Tag | GLE-H52H3-1mg| Gentaur Distribution US, UK & Europe

Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose. Thereby, it plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides, it participates in the PKC-activated salvage pathway of ceramide formation and plays a role in cholesterol metabolism. It may either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol. Defects in Glucosylceramidase are the cause of Gaucher disease, also known as glucocerebrosidase deficiency. Gaucher disease is the most prevalent lysosomal storage disease, characterized by accumulation of glucosylceramide in the reticulo-endothelial system. Currently, enzyme replacement therapy is used to treat patients with the disease.

Source: Human Glucosylceramidase, His Tag (GLE-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 40 - Gln 536 (Accession # P04062-1).

Format: Liquid.

Tag: C-10×His.

Expression System: HEK293.

Expression Region: Ala 40 - Gln 536.

Conjugate: Unconjugated.

Molecular Weight: 57.5 kDa.

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 57.5 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: 90%.

Buffer: 0.1 M Sodium Citrate, 1 mM TCEP, pH5.5.

Storage Conditions: -70℃.

Shipping Conditions: Dry ice