Human EAR2(Eosinophil Associated Ribonuclease A family Member 2) ELISA Kit | E-EL-H1943

Human EAR2(Eosinophil Associated Ribonuclease A family Member 2) ELISA Kit | E-EL-H1943 | Gentaur US, UK & Europe Disrtribition

Target Species: Human

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivitys: 0.1ng/mL

Detection Range: 0.16~10ng/mL

Target Name: EAR2

Target Synonyms: N/A

Tested Sample: Serum, plasma and other biological fluids

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EAR2. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EAR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EAR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human EAR2. You can calculate the concentration of Human EAR2 in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Human EAR2 in samples. No significant cross-reactivity or interference between Human EAR2 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Human EAR2 concentrations in Serum, plasma and other biological fluids.

Research Area: Epigenetics and Nuclear Signaling, Immunology

Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.