Description
Human CD73-coupled Magnetic Beads | MBS-K022-10mg| Gentaur Distribution US, UK & Europe
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Source: Human CD73 Protein is expressed from human 293 cells (HEK293) . It contains AA Trp 27 - Ser 549 (Accession # P21589-1) .
Molecule: CD73.
Format: Powder.
Species: Human.
Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
Kit Component: Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
Stability: Upon receipt, please store the beads at -20°C for 1 year in lyophilized state. Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
Storage Conditions: -20℃, aviod light.
Shipping Conditions: RT
