HT22 Cell Line | CL-0697

HT22 Cell Line | CL-0697 | Gentaur US, UK & Europe Disrtribition

Media: CM-0697

Organism: Mus musculus, mouse

Application: N/A

Background: HT-22 is an immortalized mouse hippocampal cell line subcloned from the HT-4 cell line (1). The parental HT-4 cell line was derived from the immortalization of mouse neuronal tissues with a temperature sensitive SV40 T-antigen (2). HT-22 is highly sensitive to glutamate and is thus frequently used as a model system to study glutamate-induced toxicity in neuronal cells.

Age: N/A

Tissue: Brain; hippocampus

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: DMEM(PM150210)+10%FBS+1%P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:6

Renewal: N/A

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days