Histone H3 Antibody (pSer10) | V8647SAF-100UG

Histone H3 Antibody (pSer10) | V8647SAF-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free

Format: Purified

Clone: PHH3/471R

Host: Mouse

Clonality: Recombinant Rabbit Monoclonal

Isotype: Mouse IgG2a, kappa

Species Reactivity: Human

Application: IHC-P

Application Details: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT

Application Note: Optimal dilution of the recombinant PHH3 antibody should be determined by the researcher.

Purity: Protein G affinity chromatography

Description: Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3 was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.

Immunogen: A synthetic peptide corresponding to the amino acids surrounding phospho S10 were used as the immunogen for the recombinant PHH3 antibody.

Storage: Store the recombinant PHH3 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Nucleus